DNA Extraction and Purification
Zymo Research Corporation Quick-DNA™ MiniPrep (200 Preps) w/ Zymo-Spin™ IIC Columns (Capped)
For easy, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits. Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
Zymo Research Corporation Oligo Clean & Concentrator (50 Preps)
There is no need for organic denaturants or chloroform. Instead, the kit features Zymo-Spin column technology and employs a single-buffer system that allows for efficient oligonucleotide adsorption to the matrix of Zymo-Spin™ IC Column. Oligonucleotide is washed and concentrated into a small volume of water ( 6 l). Purified oligonucleotide, available in just 2 minutes, is suitable for hybridization, gel shift assays, enzymatic reactions, ligation, sequencing, microarray analysis, etc.
Zymo Research Corporation DNA Clean & Concentrator™-100 (50 Preps) w/ Zymo Spin V
The DCC-100 employs a single-buffer systemthat allows for efficient DNA adsorption onto the matrix of the supplied Zymo-Spin™ V Columns. Simply add the specially formulated DNA Binding Buffer to your sample and transfer the mixture to the supplied Zymo-Spin™ V Column with Reservoir. There is no need for organic denaturants or chloroform. The purified DNA is ideal for DNA ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures. The entire DNA purification/concentration procedure typically takes less than 20 minutes and can be performed using a centrifuge or vacuum source together with a microcentrifuge.
Zymo Research Corporation EZ DNA Methylation-Gold™ Kit (200 Rxns)
The EZ DNA Methylation-Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses heat denaturation instead of chemical denaturation of the input DNA. This allows for the denaturation and bisulfite conversion steps to be consolidated into one step, leading to a much-reduced incubation time. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
Zymo Research Corporation Genomic DNA Clean & Concentrator™ Kit (100 Preps)
There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied ZymoSpin™ Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. The product is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.
Zymo Research Corporation Quick-DNA Fungal/Bacterial Microprep Kit, 50 Preps
The Quick-DNA Fungal/Bacterial Microprep Kit is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.
Zymo Research Corporation Genomic DNA Clean & Concentrator™ Kit-10 (25 Preps)
There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied ZymoSpin™ Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. The product is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.
Zymo Research Corporation Quick DNA Fecal/Soil Microbe MiniPrep™ Kit (50 Preps)
The kit can be used to successfully isolate DNA from tough-to-lyse Gram-positive and Gram-negative bacteria, fungi, algae, protozoa, etc. that inhabit fecal and soil samples. The procedure is easy and can be completed in as little as 15 minutes: fecal samples (150 mg each) or soil samples (250 mg each) are added directly to a ZR BashingBead™ Lysis Tube (0.1 & 0.5 mm) and rapidly and efficiently lysed by bead beating without the use of organic denaturants or proteinases. Zymo-Spin™ Technology is then used to isolate the DNA, which is subsequently filtered to remove humic acids/polyphenols that inhibit PCR. The DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, etc.
Zymo Research Corporation Zyppy™ Plasmid Miniprep Kit (800 Preps)
Our Zyppy™ Plasmid Miniprep Kit is the fastest and simplest method available to efficiently separate plasmid DNA from E. coli. The plasmid DNA is of the highest quality, is endotoxin-free, and is well suited for use in bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications. An overview of the purification procedure is shown to the right.
Biochain Institute Inc Genomic DNA - Human Adult Normal Tissue: Adipose, from a single donor, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Zymo Research Corporation Solution 2 Lysis Buffer, 90ml
Solution 2 Lysis Buffer is used for alkaline lysis of Zymolyase-digested yeast for plasmid DNA purification.
Zymo Research Corporation EZ-96 DNA Methylation-Gold MagPrep (4x96 rxns.)
The EZ-96 DNA Methylation-Gold MagPrep integrates DNA denaturation and bisulfite conversion processes into one step followed by a magnetic bead-based clean-up for high-throughput methylation analysis. To streamline the procedure, high temperature is used instead of sodium hydroxide to denature DNA. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The EZ DNA Methylation-Gold kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for downstream analyses, including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
LAMDA BIOTECH INC Column-Pure™ Animal Genomic DNA Kit
This is a quick and easy spin column method for isolation of genomic DNA from various animal tissues and cultured cells. The kit is designed to process tissue samples up to 30mg. The isolated DNA is of high quality for many molecular biology applications, such as; PCR, restriction digestion, and other downstream applications.
Biochain Institute Inc Express Cloning Checker Kit I: Red Solution, 1 ml/PK
BioChain's Express Cloning Checker Kits provide highly efficient methods of identifying recombinant colonies after transformation eliminating time-consuming plasmid preparation. In the large-scale screen method, the partial colonies of E. coli bacteria can be directly transfered from the transformation plates into the solutions of the kits and immediately run on an agarose gel electrophoresis. The results will be known within 1-2 hours instead of the 2 days required in a conventional method of labor-intensive plasmid DNA miniprep or colony hybridization.