DNA Extraction and Purification
LifeSensors UBITEST MAGNETIC TUBE ELUTION KIT
The UbiTest kit determines whether or not a target protein is poly-ubiquitinated. This powerful platform is an improvement on the most common method for investigating protein ubiquitination: immunoprecipitation and Western blot analysis. However, the substrate antibody often interacts differently with the poly-ubiquitinated forms of the substrate in the immuno-blotting step. This is due to epitope masking, reduced affinity, or changes in selectivity. A more definitive method for demonstrating protein ubiquitination is to couple immunoprecipitation with digestion by a broad spectrum deubiquitinase (DUB) prior to immunoblot analysis. An increased signal for the protein of interest (POI) after DUB treatment is a clear indication that the protein was ubiquitinated even if there was no clear reactivity in the untreated sample.
Zymo Research Corporation MagBead DNA/RNA Wash 2, 20 ml
MagBead DNA/RNA Wash 2 for use with several Zymo Research Magnetic bead based kits for high-throughput automation of DNA and RNA purification.
![Zymo Research Corporation ZymoBIOMICS 96 DNA Kit (2 x 96 preps)Includes: [D4307] ZymoBIOMICS 96 DNA Kit x 1, [S6012-50] ZR BashingBead Lysis Tubes (0.1mm & 0.5 mm) 50 pack x 4[D4300-1-150] ZymoBIOMICS Lysis Solution, 150 ml x 1](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502037104.JPG-250.jpg)
Zymo Research Corporation ZymoBIOMICS 96 DNA Kit (2 x 96 preps)Includes: [D4307] ZymoBIOMICS 96 DNA Kit x 1, [S6012-50] ZR BashingBead Lysis Tubes (0.1mm & 0.5 mm) 50 pack x 4[D4300-1-150] ZymoBIOMICS Lysis Solution, 150 ml x 1
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
Zymo Research Corporation EZ-96 DNA Methylation-Lightning MagPrep (8x96 rxns.)
The EZ-96 DNA Methylation-Lightning MagPrep Kit provides high-throughput and automated methylation analysis. No preparation is necessary with the ready-to-use conversion reagent - simply add this unique reagent to a DNA sample and incubate the reaction for about one hour. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. This kit produces the highest yields of bisulfite-converted DNA that is ideal for PCR, arrays, library preps, and Next-Generation sequencing.
Zymo Research Corporation Quick-DNA Plant/Seed 96 Kit (2 x 96 Preps)
The procedure is easy and can be completed in as little as 50 minutes: plant samples ( 80 mg each) are added directly to the tubes of a ZR BashingBead™ Lysis Rack (2.0 mm) and are rapidly and efficiently lysed by bead beating (e.g., 2010 GenoGrinder Instrument, page 5) without the use of organic denaturants or proteinases. Polysaccharides and polyphenols/tannins are removed from the DNA using our Zymo-Spin™ 96-well plate and Silicon-A™-HRC Plate technologies, respectively. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc.
New England Biolabs, Inc. Monarch® HMW DNA Extraction Kit for Tissue - 5 preps
The Monarch HMW DNA Extraction Kit for Tissue provides a rapid and reliable process for extracting high molecular weight (HMW), intact genomic DNA from various tissues and bacteria, as well as other sample types including yeast, insect, and amphibian. Optimized extraction protocol for tissue utilizes pestle homogenization and proteinase K digestion with agitation for sample lysis, followed by a protein removal step and precipitation of the extracted DNA onto the surface of large glass beads. DNA size ranges from 50 to greater than or equal to 500 kb for standard protocols and into the Mb range when the lowest agitation speeds are used for processing soft organ tissues and bacteria. Purified DNA is recovered in high yield with excellent purity, including nearly complete removal of RNA. Purified HMW DNA is suitable for a variety of downstream applications including long-read sequencing
Zymo Research Corporation S&P DNA Binding Buffer, 170 ml
A component for use with the Quick-cfDNA Serum & Plasma Kit
Zymo Research Corporation Solid Tissue Digestion Buffer II, 22 ml
Solid Tissue Digestion Buffer II for use with Quick-DNA purification line from Zymo Research. Sold separately or as part of a complete purification kit.
Zymo Research Corporation ZymoBIOMICS DNase/RNase Free Water, 30 ml
ZymoBIOMICS™ MagBinding Beads 3 mL
Zymo Research Corporation Human WGA Methylated DNA (5 μg / 20 μl)
The Human WGA Methylated & Non-methylated DNA Set isgenerated using phi29 DNA polymerase based whole genome amplification techniques from HCT116 DKO cell line derived genomic DNA (Human HCT116 DKO Non-methylated DNA). The Human WGA Methylated DNA is Human WGA Non-methylated DNA that has been enzymatically methylated at all double-stranded CGdinucleotides using M.SssI methyltransferase and can be used as a positive control for DNA methylation analysis.